Robbers wielding a crowbar hit the Golden Mile Tavern in Templeton in the early hours of Saturday morning taking an unknown amount of cash.
Two men entered the Trents Rd pub at about 2am and threatened staff before stealing a quantity of cash from the office area, police said.
Staff were unhurt in the robbery, but are very shaken up, an employee said.
Acting Detective Sergeant Matt Reynolds said good CCTV footage from the tavern shows the offenders during the robbery.
Anyone who may have information about the robbery, or who recognises the clothing worn by the offenders, can phone 363 7400 and quote file number 170715/7675.
Alternatively, information can be passed through Crimestoppers anonymously on 0800 555 111.